Johann Peter Griess 6 September — 30 August was an industrial chemist [1] and an early pioneer of organic chemistry. After he finished at an agricultural private school, he joined the Hessian cavalry , but left the military shortly after. He started his studies at the University of Jena in , but changed to the University of Marburg in During his student life he was several times sentenced to the Karzer campus jail and was also banned from the city for one year, during which time he listened to lectures of Justus Liebig at the Ludwig Maximilian University of Munich. After most of the family possession had been spent, he had to start working at the chemical factory of Oehler in Offenbach am Main in

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The identification of nitrite in saliva has been the first analytical application of this diazotization reaction in For a century, the Griess reaction has been exclusively used to identify analytically bacterial infection in the urogenital tract, i.

The Griess reaction is specific for nitrite. Analysis of nitrate by this reaction requires chemical or enzymatic reduction of nitrate to nitrite prior to the diazotization reaction. Today, we know dozens of assays based on the Griess reaction. In principle, every laboratory in this area uses its own Griess assay.

The simplest Griess assay is performed in batch commonly as originally reported by Griess. Because of the recognition of numerous interferences in the analysis of nitrite and nitrate in biological fluids and of the desire to analyze these anions simultaneously, the Griess reaction has been repeatedly modified and automated.

The present article gives an overview of the currently available assays of nitrite and nitrate in biological fluids based on the Griess reaction. Special emphasis is given to human plasma and urine, to quantitative aspects, as well as to particular analytical and pre-analytical factors and problems that may be associated with and affect the quantitative analysis of nitrite and nitrate in these matrices by assays based on the Griess reaction. This site needs JavaScript to work properly.

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Send even when there aren't any new results. Optional text in email:. Save Cancel. Create a file for external citation management software Create file Cancel. Full-text links Cite Favorites. Similar articles Methods of quantitative analysis of the nitric oxide metabolites nitrite and nitrate in human biological fluids. Tsikas D. Free Radic Res. PMID: Review. Romitelli F, et al. Epub Feb PMID: Measurement of nitric oxide synthase activity in vivo and in vitro by gas chromatography-mass spectrometry.

Methods Mol Biol. Analysis of nitrite and nitrate in biological samples using high-performance liquid chromatography. Jobgen WS, et al. Epub Aug Measurement of nitrite and nitrate in biological fluids by gas chromatography-mass spectrometry and by the Griess assay: problems with the Griess assay--solutions by gas chromatography-mass spectrometry. Tsikas D, et al. Anal Biochem. Show more similar articles See all similar articles. Aranke M, Mian AI. Aranke M, et al. Clin Pediatr Emerg Med.

Epub Jun Hou J, et al. Sensors Basel. Farhadi Moghadam B, Fereidoni M. Farhadi Moghadam B, et al. PLoS One. Dogaru G, et al. In Vivo. Antioxidant molecular mechanism of adenosyl homocysteinase from cyanobacteria and its wound healing process in fibroblast cells. Sarkar P, et al. Mol Biol Rep. Epub Jan Show more "Cited by" articles See all "Cited by" articles. Publication types Review Actions.

MeSH terms Animals Actions. Humans Actions. Substances Nitrates Actions. Nitrites Actions. Nitric Oxide Actions. Arginine Actions. Full-text links [x] Elsevier Science. Copy Download.


Peter Griess






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