Online First. Sudharto, Tembalang, Semarang, , Indonesia. African Journal of Microbiology Research, 2 7 , Boam, A.

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Antioxidant and antimicrobial activities of clove bud essential oil and eugenol nanoparticles in alcohol-free microemulsion. Clove bud essential oil CEO and its major individual phenolic constituent eugenol were formulated as nanoparticles in water-based microemulsion systems.

The oil titration method was used to incorporate different amounts of the oil and eugenol in the micellar solution of Tween Results showed that microemulsion improved the evaluated activities of CEO and eugenol compared with the crude counterparts. Individual eugenol microemulsion was more effective than CEO microemulsion which contained only The results of this study could have potential applications in water-based disinfectants, preservation and flavoring of food and in personal hygiene products.

It may also have promising applications in the nutraceutical and functional beverage field. Enterohemorrhagic Escherichia coli OH7 EHEC has caused foodborne outbreaks worldwide and the bacterium forms antimicrobial-tolerant biofilms.

We investigated the abilities of various plant essential oils and their components to inhibit biofilm formation by EHEC. Bay, clove, pimento berry oils and their major common constituent eugenol at 0. In addition, three other eugenol derivatives isoeugenol, 2-methoxypropylphenol, and 4-ethylguaiacol had antibiofilm activity, indicating that the C-1 hydroxyl unit, the C-2 methoxy unit, and C-4 alkyl or alkane chain on the benzene ring of eugenol play important roles in antibiofilm activity.

Interestingly, these essential oils and eugenol did not inhibit biofilm formation by three laboratory E. Transcriptional analysis showed that eugenol down-regulated 17 of 28 genes analysed, including curli genes csgABDFG , type I fimbriae genes fimCDH and ler-controlled toxin genes espD, escJ, escR, and tir , which are required for biofilm formation and the attachment and effacement phenotype.

In addition, biocompatible poly lactic-co-glycolic acid coatings containing clove oil or eugenol exhibited efficient biofilm inhibition on solid surfaces. Gastroprotective activity of essential oil of the Syzygium aromaticum and its major component eugenol in different animal models. Syzygium aromaticum, a medicinal plant commonly known as clove, is used to treat toothache, respiratory disorders, inflammation, and gastrointestinal disorders.

From the flower buds of S. The aims of this study were: 1 to extract the essential oil of the flower buds of S.

Both essential oils from S. Studies focusing on the possible mechanisms of gastroprotection were also undertaken using the following experiments: evaluation of gastric secretion by the pylorus-ligated model, determination of mucus in gastric content, participation of nitric oxide NO and endogenous sulfhydryl in gastric protection.

The results show that there was no significant effect on the volume of gastric juice and total acidity. However, the quantification of free gastric mucus showed that the clove oil and eugenol were capable of significantly enhancing mucus production. With regard to the NO and endogenous sulfhydryls, the results demonstrated that the gastroprotection induced by clove oil and eugenol are not related to the activities of the nitric oxide and endogenous sulfhydryls.

No sign of toxicity was observed in the acute toxicity study. In conclusion, the results of this study show that essential oil of S. The data suggest that the effectiveness of the essential oil and eugenol is based on its ability to stimulate the synthesis of mucus, an.

Anti-inflammatory and antinociceptive activities A of eugenol essential oil in experimental animal models. Directory of Open Access Journals Sweden.

Full Text Available Eugenia caryophyllata, popular name "clove", is grown naturally in Indonesia and cultivated in many parts of the world, including Brazil. Clove is used in cooking, food processing, pharmacy; perfumery, cosmetics and the clove oil eugenol have been used in folk medicine for manifold conditions include use in dental care, as an antiseptic and analgesic.

The objective of this study was evaluated the anti-inflammatory and antinociceptive activity of eugenol used for dentistry purposes following oral administration in animal models in vivo. The anti-inflammatory activity of eugenol was evaluated by inflammatory exudates volume and leukocytes migration in carrageenan-induced pleurisy and carrageenan-induced paw edema tests in rats.

The antinociceptive activity was evaluated using the acetic acid-induced writhing and hot-plate tests in mice. The data suggest that eugenol possesses anti-inflammatory and peripheral antinociceptive activities.

Acaricidal activity of essential oil of Syzygium aromaticum, hydrolate and eugenol formulated or free on larvae and engorged females of Rhipicephalus microplus. The cattle tick, Rhipicephalus microplus Canestrini, Ixodida: Ixodidae , is the most important ectoparasite in cattle-breeding areas and is responsible for severe economic losses.

Synthetic acaricides have been used to control this parasite. However, the need for safer products has stimulated the search for new acaricides, such as those to be obtained from medicinal plants. The essential oil of Syzygium aromaticum clove has many biological properties and shows great potential for use in veterinary applications. In the context of the need for new agents, this study investigated the in vitro properties of the hydrolate, essential oil and the main constituent of S.

The hydrolate showed no activity. The results of these tests reaffirm the important potential of clove essential oil and eugenol. Assessment of antioxidative, chelating, and DNA-protective effects of selected essential oil components eugenol , carvacrol, thymol, borneol, eucalyptol of plants and intact Rosmarinus officinalis oil.

Selected components of plant essential oils and intact Rosmarinus officinalis oil RO were investigated for their antioxidant, iron-chelating, and DNA-protective effects. Antioxidant activities were assessed using four different techniques. It was observed that whereas eugenol , carvacrol, and thymol showed high antioxidative effectiveness in all assays used, RO manifested only antiradical effect and borneol and eucalyptol did not express antioxidant activity at all. DNA-protective ability against hydrogen peroxide H2O2 -induced DNA lesions was manifested by two antioxidants carvacrol and thymol and two compounds that do not show antioxidant effects RO and borneol.

This paper evaluates the results in the light of experiences of other scientists. Large-scale preparation of clove essential oil and eugenol -loaded liposomes using a membrane contactor and a pilot plant. Based on our previous study where optimal conditions were defined to encapsulate clove essential oil CEO into liposomes at laboratory scale, we scaled-up the preparation of CEO and eugenol Eug -loaded liposomes using a membrane contactor mL and a pilot plant 3 L based on the principle of ethanol injection method, both equipped with a Shirasu Porous Glass membrane for injection of the organic phase into the aqueous phase.

Homogenous, stable, nanometric-sized and multilamellar liposomes with high phospholipid, Eug loading rates and encapsulation efficiency of CEO components were obtained. Saturation of phospholipids and drug concentration in the organic phase may control the liposome stability.

Liposomes loaded with other hydrophobic volatile compounds could be prepared at large scale using the ethanol injection method and a membrane for injection. Plant oils thymol and eugenol affect cattle and swine waste emissions differently. Wastes generated from the production of cattle and swine in confined facilities create the potential for surface and groundwater pollution, emission of greenhouse gases, transmission of pathogens to food and water sources, and odor.

It is our hypothesis that something which inhibits microbial fermentation in livestock wastes will be beneficial to solving some of the environmental problems. Our work has concentrated on the use of antimicrobial plant oils , thymol, thyme oil , carvacrol, eugenol and clove oil. Anaerobic one-litre flasks with a working volume of 0. Either oil at 0. In cattle but not swine waste, thymol prevented the accumulation of lactate. However, eugenol stimulated lactate formation in cattle and swine wastes.

Thus, eugenol may offer a distinct advantage over thymol, because lactate accumulation in the wastes causes the pH to drop more rapidly, further inhibiting microbial activity and nutrient emissions. We conclude that plant oils may offer solutions to controlling various environmental problems associated with livestock wastes, assuming that they are cost-effective.

Chemical composition of essential oils of Eugenia caryophylla and Results: The essential oils obtained yielded of 5.

Phytochemical and antimicrobial studies on essential oils of some Jul 12, Key words: Essential oils , aromatic plants, phytochemical analysis, antimicrobial activity.

A potential study on clove oil , eugenol and eugenyl acetate as diesel fuel bio-additives and their performance on one cylinder engine. Full Text Available Research on the potency of essential oils as diesel fuel bio-additives has been reported.

It also has been found out that clove oil has a better performance than turpentine oil on decreasing Break Specific Fuel Consumption BSFC and reduces the exhaust emissions of the engine. Clove oil is essential oil the content of which is made of eugenol acting as the main component. Eugenol has a bulky structure, two oxygen atoms and can form eugenyl acetate from ester reaction. Eugenyl acetate has a bulkier structure and higher oxygen content than eugenol which leads to optimizing the process of fuel combustion.

This experiment can give information about the potency of the bio-additive based on clove oil and eugenol and about the influence of oxygen enrichment with eugenol on the performance of the diesel fuel bio-additive. In general, this experiment covered three stages.

The second step is the characterization of the diesel fuel bio-additive and composition optimization. The final step is conducting a diesel fuel bio-additive performance test on one cylinder engine on a laboratory scale. The results of the carried out experiment show that clove oil , eugenol and eugenyl acetate can decrease Break Specific Fuel Consumption BSFC and reduce the exhaust emissions of the engine as well as oxygen enrichment can help in reaching optimal fuel combustion. The separation technology such as distillation and absorption are well known in essential oil purification.

The purification of clove leaf oil needs an attention because the current technology still consumes high energy and produces chemical wastes. The employment of membrane separation for clove leaf purification is a novel concept that needs many improvements.

The main problem of polymeric membrane utilization is eugenol ability to dissolve the polymer membrane. Cellulose acetate is one of membrane polymers that is insoluble in eugenol. The stability of produced membrane as an organic solvent nanofiltration OSN is evaluated in this study. The SEM image result shows that fabricated membrane has an asymmetric structure of membrane sub-layer. The different nano-particles loading shows the variation of permeate fluxes, the increase of nano-particles in polymer blend tends to increase the permeability.

Essential oils are potential alternatives to synthetic insecticides because they have low mammalian toxicity, degrade rapidly in the environment, and possess complex mixtures of bioactive constituents with multi-modal activity against the target insect populations. Twenty-one essential oils were initially screened for their toxicity against Aedes aegypti L.

Manuka essential oil interacted synergistically with oregano essential oil and antagonistically with clove bud essential oil. GC-MS analysis revealed the presence of 21 components in Manuka essential oil and three components each in oregano and clove bud essential oils. Eugenol Manuka essential oil interacted synergistically with eugenol acetate and antagonistically with eugenol , suggesting that eugenol was a major contributor to the antagonistic interaction between Manuka and clove bud essential oils.

In addition, Manuka interacted synergistically with carvacrol suggesting its contribution to the synergistic interaction between Manuka and oregano essential oils. These findings provide novel insights that can be used to develop new and safer alternatives to synthetic insecticides.

Comparative study of the antifungal activity of some essential oils This study aimed to evaluate the antimould activity of oregano, thyme, rosemary and clove essential oils and some of their main constituents: eugenol , carvacrol and thymol against Aspergillus niger.

This antifungal activity was assessed using broth dilution, disc diffusion and micro atmosphere methods. In both agar diffusion


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