In this automated apoenzyme reactivation immunoassay system Ames Optimate for thyroxin-binding globulin TBG , the sample and N6-aminohexylflavin adenine dinucleotide-labeled TBG react sequentially with antiserum. Then apoglucose oxidase is added to combine with the free fraction and generate glucose oxidase activity, which is measured colorimetrically. The assay requires microL of sample and covers the clinically significant range for TBG less than 2. The first result is obtained in 16 min; assay of 29 samples and their blanks is completed in less than 1 h.
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We assessed the performance of the apoenzyme reactivation immunoassay system ARIS reagent strip tests for determination of phenobarbital PB and phenytoin PHT with the Seralyzer reflectance photometer. In the assay, the drug of the sample competes with a flavine adenine dinucleotide FAD -drug conjugate for binding to a specific antibody; the unbound conjugate then activates apoglucose oxidase to reconstitute glucose oxidase, whose activity is kinetically monitored by a coupled chromogenic reaction.
Within-run coefficients of variation CVs were less than or equal to 5. Mean analytical recoveries were These homogeneous competitive colorimetric immunoassays are particularly suitable for emergency use, for testing small batches of samples, wherever prompt results are needed.
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Similar articles Evaluation of the Ames Seralyzer for the determination of carbamazepine, phenobarbital, and phenytoin concentrations in saliva. Miles MV, et al. Ther Drug Monit. PMID: Quantitative determination of phenytoin and phenobarbital in capillary blood by Ames Seralyzer. Graves NM, et al. Determination of serum theophylline by apoenzyme reactivation immunoassay system.
Plebani M, Burlina A. Plebani M, et al. Dry-reagent strips for measuring phenytoin in serum. Sommer R, et al. Clin Chem. Dry reagent immunoassay for measuring phenobarbital in serum and plasma.
Show more similar articles See all similar articles. Cited by 1 article The use of solid-phase chemistry in therapeutic drug monitoring. Mould G, Marks V. Mould G, et al. Clin Pharmacokinet. PMID: Review. No abstract available. MeSH terms Apoenzymes Actions. Colorimetry Actions. Evaluation Studies as Topic Actions. Glucose Oxidase Actions. Humans Actions.
Immunoenzyme Techniques Actions. Reagent Strips Actions. Regression Analysis Actions. Substances Apoenzymes Actions. Phenytoin Actions. Phenobarbital Actions. Copy Download.
Homogeneous Apoenzyme Reactivation Immunoassay for Thyroxin-Binding Globulin in Serum
This application is a continuation in part of, and claims the priority benefit of, non-provisional application Ser. This application is also a continuation in part of, and claims the priority benefit of, copending U. Copending application Ser. The field of the invention is improved electrochemical diagnostic reagents useful for instrumented tests for coagulation, immunoassays, and other analytes.
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